Journal article
Adhiraj Roy, Y. Shin, Jeong‐Ho Kim
Analytical Biochemistry, 2013
Analytical Biochemistry, 2013
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APA
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Roy, A., Shin, Y., & Kim, J. H. (2013). Construction of yeast strains useful for screening drugs that inhibit glucose uptake and glycolysis. Analytical Biochemistry.
Chicago/Turabian
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Roy, Adhiraj, Y. Shin, and Jeong‐Ho Kim. “Construction of Yeast Strains Useful for Screening Drugs That Inhibit Glucose Uptake and Glycolysis.” Analytical Biochemistry (2013).
MLA
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Roy, Adhiraj, et al. “Construction of Yeast Strains Useful for Screening Drugs That Inhibit Glucose Uptake and Glycolysis.” Analytical Biochemistry, 2013.
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@article{adhiraj2013a,
title = {Construction of yeast strains useful for screening drugs that inhibit glucose uptake and glycolysis},
year = {2013},
journal = {Analytical Biochemistry},
author = {Roy, Adhiraj and Shin, Y. and Kim, Jeong‐Ho}
}
Abstract
The budding yeast Saccharomyces cerevisiae expresses different isoforms of glucose transporters (HXTs) in response to different levels of glucose. Here, we constructed reporter strains in which the nourseothricin (NAT) resistance gene is expressed under the control of the HXT1, 2, or 3 promoter. The resulting HXT-NAT reporter strains exhibited a strict growth dependence on glucose, and their growth could be easily controlled and optimized by adjusting glucose concentration, demonstrating the value of the reporter strains for studying the molecular basis of differential expression of HXT genes, as well as for screening drugs that inhibit glucose uptake and glycolysis.