Functional dissection of the glucose signaling pathways that regulate the yeast glucose transporter gene (HXT) repressor Rgt1

Journal article

David J. Jouandot, Adhiraj Roy, Jeong‐Ho Kim
Journal of Cellular Biochemistry, 2011

Semantic Scholar DOI PubMedCentral PubMed

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APA Click to copy
Jouandot, D. J., Roy, A., & Kim, J. H. (2011). Functional dissection of the glucose signaling pathways that regulate the yeast glucose transporter gene (HXT) repressor Rgt1. Journal of Cellular Biochemistry.

Chicago/Turabian Click to copy
Jouandot, David J., Adhiraj Roy, and Jeong‐Ho Kim. “Functional Dissection of the Glucose Signaling Pathways That Regulate the Yeast Glucose Transporter Gene (HXT) Repressor Rgt1.” Journal of Cellular Biochemistry (2011).

MLA Click to copy
Jouandot, David J., et al. “Functional Dissection of the Glucose Signaling Pathways That Regulate the Yeast Glucose Transporter Gene (HXT) Repressor Rgt1.” Journal of Cellular Biochemistry, 2011.

BibTeX Click to copy

@article{david2011a,
  title = {Functional dissection of the glucose signaling pathways that regulate the yeast glucose transporter gene (HXT) repressor Rgt1},
  year = {2011},
  journal = {Journal of Cellular Biochemistry},
  author = {Jouandot, David J. and Roy, Adhiraj and Kim, Jeong‐Ho}
}

Abstract

The yeast Rgt1 repressor is a bifunctional protein that acts as a transcriptional repressor and activator. Under glucose‐limited conditions, Rgt1 induces transcriptional repression by forming a repressive complex with its corepressors Mth1 and Std1. Here, we show that Rgt1 is converted from a transcriptional repressor into an activator under high glucose conditions and this occurs through two independent but consecutive events mediated by two glucose signaling pathways: (1) disruption of the repressive complex by the Rgt2/Snf3 pathway; (2) phosphorylation of Rgt1 by the cAMP‐dependent protein kinase (cAMP‐PKA) pathway. Rgt1 is phosphorylated by PKA at four serine residues within its amino‐terminal region, but this does not occur until the repressive complex is disrupted. While phosphorylation of any one of these sites is sufficient to enable Rgt1 to induce transcriptional activation, phosphorylation of all the sites results in the release of Rgt1 from DNA. We discuss how the bifunctional properties of Rgt1 are regulated through differential phosphorylation. J. Cell. Biochem. 112: 3268–3275, 2011. © 2011 Wiley Periodicals, Inc.